Crystallization of Biological Macromolecules, by Alexander McPherson. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory. Crystals of macromolecules have become keystones of molecular biology, bridging Chapter 3: The Purification and Characterization of Biological Macromolecules ~xray/Books/ Results 1 – 19 of 19 Crystallization of Biological Macromolecules by Alexander McPherson and a great selection of related books, art and collectibles available.

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Contents The history and character of macromolecular crystals; principles of macromolecular structure and the applications of x-ray crystallography; the purification and macromoleculfs of biological macromolecules; some physical and energetic principles; practical procedures for macromolecular crystallization; important considerations in macromolecular crystallization; strategies and special approaches in growing crystals; impurities, defects, and crystal quality; the mechanisms and kinetics of macromolecular crystal growth; crystal growth in unique environments.

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Crystallization of Biological Macromolecules

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Benton Richard Fortey View All. The quality of an X-ray structure determination is directly correlated with the size and the biologiacl of the crystalline samples; thus, refinement of conditions should always be a primary component of crystal growth. Customer Reviews Review this book.

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The improvement process is referred to as optimization, and it entails sequential, incremental changes in the chemical parameters that influence crystallization, such as pH, ionic strength crystallizatuon precipitant concentration, as well as physical parameters such as temperature, sample volume and overall methodology. Here, an attempt is made to provide guidance macroolecules how optimization might best be applied to crystal-growth problems, and what parameters and factors might most profitably be explored to accelerate and achieve success.

Have a Nice DNA. It also includes the application of some unique procedures and approaches, and the addition of novel components such as detergents, ligands or other small molecules that may enhance nucleation or crystal development.

Exceptional customer service Get specialist help and advice. Go to British Wildlife. Even when the initial samples are suitable, often marginally, refinement of conditions is recommended in order to obtain the highest quality crystals that can be grown.

Crystallization of biological macromolecules ( edition) | Open Library

Go to Conservation Land Management. Praise of Chromosome Folly. For the successful X-ray structure determination of macromolecules, it is first necessary to identify, usually by matrix screening, conditions that yield some sort of crystals. British Wildlife is the leading natural history magazine in the UK, providing essential reading for both enthusiast and professional naturalists and wildlife conservationists.

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Crystallization of biological macromolecules for X-ray diffraction studies.

This extensively illustrated book provides a biochemical context in macromoleculed crystal growth can be pursued and instructs readers in practical aspects of the technology, laying out effective strategies for success.

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Crystallization of Biological Macromolecules

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It is therefore generally necessary to improve upon these initial conditions in order to obtain better crystals of sufficient quality for X-ray data collection.

Initial crystals are frequently microcrystals or clusters, and often have unfavorable morphologies or yield poor diffraction intensities. About Help Blog Jobs Welcome to crystzllization new website.

About this book This extensively illustrated book provides a biochemical context in which crystal growth can be pursued and instructs readers in practical biologucal of the technology, laying out effective strategies for success.